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First published online 5 November 2003
doi: 10.1242/dev.00856

Development 130, 6201-6208 (2003)
Published by The Company of Biologists 2003
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Meiotic transmission of epigenetic changes in the cell-division factor requirement of plant cells

Frederick Meins, Jr* and Monique Thomas

The Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland



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  		Fig. 1. The lineage of cell clones and plants regenerated from C– leaf and C– pith tissues. Clones were obtained by plating cell suspensions in soft agar and marking colonies of single-cell origin. C– leaf clones L113N and L201N and C– pith clone P278N were derived from different plants in separate experiments. C+ variants were obtained by selecting for growth on low-cytokinin medium. S0 generation plants regenerated from subclones were selfed to obtain the S1 generation. The patterns of segregation for the C+ leaf phenotype are shown in Table 1. The origins of homozygous mutant lines Hl-1/Hl-2 and Hl-2/Hl-2 derived from subclones L113H-21 and P278H-12, respectively, are indicated. Clones showing the C+ phenotype are shaded.

 


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  		Fig. 2. Segregation of the Hl trait in plants derived from clones of Hl-2/Hl-2 leaf tissues cultured on high-cytokinin medium. Results are shown for three representative S0 plants derived from three clones. C+ cloned lines and S0, S1 and S2 plants showing the Hl trait are shaded. The numbers of C– and C+ progeny obtained in crosses are indicated below the seedling. The R values obtained and results for additional regenerates are shown in Table 7.

 




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