doi: 10.1242/10.1242/dev.00329
Macrophage stimulating protein is a target-derived neurotrophic factor for developing sensory and sympathetic neurons
Alison Forgie1,2,
Sean Wyatt1,2,
Pamela H. Correll3 and
Alun M. Davies1,2,*
1 Department of Preclinical Veterinary Sciences, Royal (Dick) School of
Veterinary Studies, Summerhall Square, Edinburgh EH9 1QH, Scotland
2 Rinat Neuroscience Corporation, 3155 Porter Drive, Palo Alto, CA 94304-1213,
USA
3 Department of Veterinary Science, Pennsylvania State University, University
Park, PA 16802-3500, USA
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Fig. 1. Comparison of the effects of MSP and NGF on the survival of DRG neurons
during development. Bar charts of the percent survival of E12 and P1 DRG
neurons after 48 hours incubation in defined medium alone (Cont) or medium
supplemented with 10 ng/ml MSP, 10 ng/ml NGF or MSP plus NGF. The means and
standard errors for data obtained from three petri dishes for each
experimental condition are shown.
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Fig. 2. Developmental changes in the dose responses of DRG neurons to MSP. Graph of
the percent survival of E12, E16, E18 and P1 DRG neurons after 48 hours
incubation with a range of concentrations of MSP. The means and standard
errors for data obtained from three petri dishes for each experimental
condition are shown.
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Fig. 3. Comparison of the effects of MSP and NGF on the survival of trigeminal
ganglion neurons during development. Percent survival of E12, E16, E18 and P1
trigeminal ganglion neurons after 48 hours incubation in defined medium alone
(Control) or medium supplemented with 10 ng/ml MSP, 10 ng/ml NGF or MSP plus
NGF. The means and standard errors for data obtained from three petri dishes
for each experimental condition are shown.
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Fig. 4. Comparison of the effects of MSP and NGF on the survival of SCG neurons
during development. Percent survival of E14, E17 and P1 SCG neurons after 48
hours incubation in defined medium alone (Control) or medium supplemented with
10 ng/ml MSP, 10 ng/ml NGF or MSP plus NGF. Graph of the percent survival of
E14 SCG neurons after 48 hours incubation with a range of concentrations of
MSP. The means and standard errors for data obtained from three petri dishes
for each experimental condition are shown.
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Fig. 5. The total length and number of branch points in the neurite arbors growing
from P1 DRG neurons after 24 hours incubation with NGF alone or NGF plus MSP
(both at 10 ng/ml). The means and standard errors of the data from three
separate experiments for length measurements and means and standard errors of
branch point counts from 110 neurons in each condition are shown.
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Fig. 6. Developmental changes in the expression of MSP mRNA in the trigeminal
ganglion and SCG, and in the maxillary target field and iris at different
developmental stages. The level of MSP mRNA relative to GAPDH mRNA in the
maxillary process at E12 and the mystacial whisker pad that develops from it
at E14, E16, E18 and P1. The level of MSP mRNA in whole trigeminal ganglia is
also shown at each of these ages. The mean and standard error of at least
three data points at each age are shown.
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Fig. 7. Total numbers of neurons in P1 and P6 trigeminal ganglia and P6 nodose
ganglia of Ron+/+ and Ron-/- mice. The
mean and standard errors are shown (n=4 wild type at both ages;
n=4 Ron-/- at both ages).
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© The Company of Biologists Ltd 2003
