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First published online 25 August 2004
doi: 10.1242/dev.01343

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Developmental modulation of Fab-7 boundary function

Department of Molecular Biology, Princeton University, Princeton, NJ 08540, USA

View larger version (58K): [in a new window]   Fig. 1. (A) Map of the Fab-7 region showing the minor nuclease hypersensitive site (*), HS1, HS2 and HS3. GAGA sites in HS1 are indicated by colored ovals and numbers 1-6. (B) Left: map of iab-7bluetail, iab6,7P6.1, iab6,7P18.1 and iab6,7P14.1 mutations showing the bluetail transposon inserted at the distal edge (right) of Fab-7 in between HS2 and HS3. The size of the Fab-7 deletion is indicated for each mutant. Right: lacZ expression in representative germband extended embryos heterozygous for iab-7bluetail, iab6,7P6.1, iab6,7P18.1 and iab6,7P14.1. Arrow in each embryo marks the anterior edge of PS12.

View larger version (65K): [in a new window]   Fig. 2. (A) Map of the Fab-7 boundary and the various HS1 subfragments used in the transgene assays. The virilis homology region is indicated by V. (B) Map of the ftz:hsp70-lacZ reporter construct. Inserts placed upstream of the ftz UPS enhancer are in the non-blocking position (NB), while inserts placed between the NE enhancer and the hsp70 promoter are in the blocking position. (C,D) lacZ expression in embryos from representative lines homozygous for ftz:hsp70-lacZ transgenes carrying different boundary elements. (C) Random DNA, five binding sites for Su(Hw) and 1.2kb Fab-7. (D) Tetramerized pHS1 fragments: pHS1x4 in the blocking position, pHS1x4 in the non-blocking position (NB), pHS1Ax4 and pHS1Bx4. All embryos in Fig. 2 (and also in Fig. 4) were stained in parallel as described elsewhere (Hagstrom et al., 1996) for a direct comparison of staining intensities.

View larger version (83K): [in a new window]   Fig. 4. (A) Map of the distal half of HS1 and the subfragments dHS1A and dHS1B. (B) lacZ expression in embryos of representative lines homozygous for ftz:hsp70-lacZ transgenes carrying different boundary elements: dHS1x4, dHS1Ax4 and dHS1Bx4.

View larger version (37K): [in a new window]   Fig. 3. (A) Map of wEN:mini-white construct containing the white enhancer (wEN), a mini-white reporter gene, and the scs' boundary element to block 3' position effects. (B) Representative adult males homozygous for transgenes containing the 1.2 kb Fab-7 element, pHS1x4, dHS1x4, dHS1Ax4 or dHS1Bx4. (C) Summary of the blocking activity seen in wEN:mini-white transgenic lines with the different boundary elements shown in Figs 2 and 4.