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Fig. 6. Neural progenitor domain patterning of feedback LDA mutants at E8.5. (A-R)
Immunofluorescence of neural tube sections from eight-to nine-somite stage
embryos. Antibodies and genotypes of the embryos as indicated in each panel.
(S-X) Analysis for ß-galactosidase activity in neural tube sections from
11- to 12-somite stage embryos. The Ptch1-null allele has the coding
sequence for nucleus-localized ß-galactosidase inserted under the
Ptch1 endogenous promoter
(Goodrich et al., 1997 ), and
the Hhip1 null allele has the coding sequence for cytoplasmic
ß-galactosidase placed under the regulation of Hhip1 promoter
(Chuang and McMahon, 1999),
allowing both to serve as potential reporters of Hh signaling. However, as
shown in T, the level of ß-galactosidase expression from the
Hhip1LacZ allele appears to be below the level of
detection, suggesting that the staining observed in U and W is predominantly
from Ptch1LacZ. (Y) Quantification of the size of each
neural progenitor population as reflected by cell numbers in wild type and
feedback LDA mutants. p3/pMN, Nkx2.2+Olig2+;
p1+p0+dorsal domain, Pax6+Nkx6.1-. (Z) Representation of
the size of each progenitor domain as a percentage of the total cell numbers
in the neural tube. Scale bar: 25 µm.
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