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First published online 19 January 2005
doi: 10.1242/dev.01647

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GATA4, 5 and 6 mediate TGFß maintenance of endodermal gene expression in Xenopus embryos

Boni Anatole Afouda^*, Aldo Ciau-Uitz and Roger Patient^,

Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK

View larger version (63K): [in a new window]   Fig. 1. Expression profiles of GATA4, 5 and 6 and their differential induction of endodermal markers in cap explants. Xenopus embryos were collected at a range of early developmental stages and total RNA was extracted for real-time RT-PCR, monitoring Sox17 (A) and GATA4-6 (B). (C) In situ hybridisation on serial sections of stages 10, 10.5 and 11 embryos, using GATA4 and 6 as probes. Arrowheads mark the dorsal blastopore lip. (D) HA-tagged Xenopus GATA4-6 synthesised in animal caps detected with rat monoclonal anti-HA antibody and anti-rat polyclonal antibody conjugated to peroxidase. LG6: long GATA6, SG6: short GATA6. G4 and G5: GATA4 and 5. Erk2 is a MAPK detected using rabbit polyclonal IgG and anti-rabbit antibody conjugated to horseradish peroxidase, as a loading control. (E,F) Induction of Sox17, HNF1ß (E) and HNF3b (F) revealed by real-time RT-PCR. Error bars represent standard deviations from the mean of three measurements of the same cDNA. Each experiment was repeated three times.

View larger version (56K): [in a new window]   Fig. 2. Testing GATA4, 5 and 6 for direct induction of endoderm markers in animal caps. Animal caps expressing HA-tagged Xenopus GATA4, 5 or 6 fused to the glucocorticoid receptor ligand-binding domain cultured in the presence or absence of dexamethasone (Dex) and/or cycloheximide (CHX) (A-C) or Emetine (EM) (D). Radiolabelled methionine incorporation measured by TCA precipitation (A) and gel electrophoresis (B). Real-time RT-PCR of Sox17, HNF1ß or HNF3ß (C,D). For error bars and Erk2 see legend to Fig. 1. (E) The data are represented as a simplified network of interactions, where Xs represent hypothetical intermediate factors, dotted lines are possibly indirect inductions and solid lines are direct inductions.

View larger version (55K): [in a new window]   Fig. 3. GATA4 induces early endoderm markers via GATA6. Stage 12 animal caps injected with HA-tagged Xenopus GATA4, 5 or 6 (50 pg) with or without GATA4, 5 or 6 MOs (5 ng). Protein production (B) and real-time RT-PCR for GATA4, 5 and 6 (A) and Sox17, HNF1ß (C,D). LG6: long GATA6, SG6: short GATA6, G5: GATA5, G4: GATA4. For Erk2 and error bars, see Fig. 1 legend. GATA factors represent some of the hypothetical intermediates (`X') in Fig. 2E (E). Xs, dotted and solid lines, as in Fig. 2 legend.

View larger version (55K): [in a new window]   Fig. 4. Activin induces Sox17 via GATA4, 5 and 6 in animal caps. Stage 12 animal caps injected with activin RNA and GATA4, 5 or 6 MOs. Real-time RT-PCR for Sox17 (A,B) or HNF1ß and endogenous GATA4, 5 and 6 (C). Data summarised in (D). Shaded box represents the temporal and/or spatial activin/nodal gradient of activity thought to develop in early embryos. Xs, dotted and solid lines as in Fig. 2 legend.

View larger version (83K): [in a new window]   Fig. 5. GATA4, 5 and 6 are required for development of the endoderm in vivo. (A,B,D) In situ hybridisation of Sox17 (A,B) and HNF1ß (D) in stage 12 embryos injected vegetally with GATA4, 5 or 6 MOs (10-40 ng). (A,D) First column shows typical whole mount images and second column shows internal staining in halved embryos, black line indicating the plane of section. Yolk plug (YP) and involuted endoderm (blue arrows) are indicated. Black arrows in (D) indicate mesodermal expression. The percentages of embryos expressing high (normal), medium or low levels of Sox17 are represented in a histogram (B). (C,E) Real time RT-PCR analysis of Sox17 (C) and HNF1ß and HNF3ß (E). For error bars see Fig. 1 legend. (F) In situ hybridisation of HNF1ß in stage 34 embryos. Position of the liver anlagen is indicated. (G) General gut morphology in stage 43 embryos. Embryos injected with 40 ng GATA6 MO did not survive to stage 43 and therefore the 20 ng phenotype is shown. The single embryo surviving up to stage 43 of the 30 ng GATA4, 5 and 6 MO injection is shown.