QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online April 13, 2005
doi: 10.1242/10.1242/dev.01792

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Takahashi, T. Articles by Bode, H. R. Search for Related Content PubMed PubMed Citation Articles by Takahashi, T. Articles by Bode, H. R. Social Bookmarking                         What's this?

Hym-301, a novel peptide, regulates the number of tentacles formed in hydra

Toshio Takahashi^1,*, Masayuki Hatta^2,, Seungshic Yum^2,, Lydia Gee¹, Masahiro Ohtani^3,, Toshitaka Fujisawa² and Hans R. Bode^1,¶

¹ Developmental Biology Center, University of California, Irvine, CA 92697, USA
² National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
³ Suntory Institute for Bioorganic Research, Osaka 618, Japan

View larger version (25K): [in a new window]   Fig. 1. (A) Nucleotide and deduced amino acid sequences of the Hym-301 gene. The predicted signal sequence is underlined and the Hym-301 is shown in red. The asterisk indicates a stop codon.

View larger version (111K): [in a new window]   Fig. 2. Expression of the Hym-301 gene (dark blue) in an adult hydra using whole-mount in situ hybridization.

View larger version (64K): [in a new window]   Fig. 3. Expression of Hym-301 gene (dark blue) during the stage of bud formation. (A) Stage 0, (B) stage 1-2, (C) stage 2-3, (D) stage 3, (E) stage 4, (F) stage 5, (G) stage 6-7 and (H) stage 8.

View larger version (103K): [in a new window]   Fig. 4. Expression of Hym-301 gene (dark blue) during head regeneration. (A) Six hours, (B) 12 hours, (C) 24 hours, (D) 48 hours, (E) 72 hours and (F) 96 hours.

View larger version (18K): [in a new window]   Fig. 5. Effect of Hym-301 on the number of tentacles formed (A), and the rate of hypostome formation (B) during head regeneration. Both figures represent regenerates treated with the Hym-301 peptide (black circles) and controls (white circles). Each data point is the average value±s.e.m. for three experiments. Fifteen to 20 regenerates were used in each of the experiments.

View larger version (17K): [in a new window]   Fig. 6. Effect of Hym-301 dsRNA on the number of tentacles formed during bud formation: Hym-301 dsRNA-treated buds (black circles), Luciferase dsRNA-treated buds (triangles) and untreated control buds (white circles). Each data point is the average value±s.e.m. for five experiments. In each experiment, 10-20 animals were used.

View larger version (35K): [in a new window]   Fig. 7. Transient reduction of the level of Hym-301 RNA in developing buds after the introduction of Hym-301 dsRNA as measured by RT-PCR. Levels of EF1- served as the control.

View larger version (101K): [in a new window]   Fig. 8. Changes in the expression pattern of Hym-301 with time following the addition of 2 mM LiCl as measured with in situ hybridization on whole mounts.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter