spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online August 14, 2006
doi: 10.1242/10.1242/dev.02496

Development 133, 3461-3471 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow A corrigendum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Development
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walser, C. B.
Right arrow Articles by Hajnal, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walser, C. B.
Right arrow Articles by Hajnal, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Distinct roles of the Pumilio and FBF translational repressors during C. elegans vulval development

Claudia B. Walser1,2,*, Gopal Battu1,*, Erika Fröhli Hoier1 and Alex Hajnal1,{dagger}

1 Zoologisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
2 Molecular Life Science PhD Program, Molekular biologisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.


Figure 1
View larger version (93K):

[in a new window]
  		Fig. 1. PUF proteins that negatively regulate vulval development. (A) Intron-exon structure and alleles of puf-8, fbf-1 and fbf-2. White boxes indicate the 5'UTRs, white boxes with arrowheads the 3'UTRs, grey boxes the coding regions and black boxes the PUF repeats. (B-E) Nomarski images of the vulval cells in L4 larvae of (B) gap-1(ga133), (C) puf-8(zh17); gap-1(ga133), and of (D,E) fbf-1(ok91) fbf-2(q704); gap-1(ga133) animals. In all panels, anterior is to the left and ventral is to the bottom. Note the ectopic induction of P4.p and P8.p (arrows in C,D,E). Arrowhead in E indicates an example of defects in the 2° cell lineage generated by P5.p resulting in the detachment of the P5.p descendants from the cuticle in a fbf-1(ok91) fbf-2(q704); gap-1(ga133) larva. Scale bar: 10 µm.

 

Figure 2
View larger version (95K):

[in a new window]
  		Fig. 2. PUF-8::GFP and FBF-2::GFP expression during vulval development. (A) Structure of the translational puf-8::gfp and fbf-2::gfp reporters. (B,D,F,H) Time-course analysis of PUF-8::GFP expression in the vulval cells from the L2 until the L4 stage with (C,E,G,J) the corresponding Nomarski images. For a semi-quantitative analysis of the expression patterns, see Fig. S1 in the supplementary material. (K,L) PUF-8::GFP expression in gonad-ablated eff-1(hy21) animals, and the corresponding Nomarski image. Note that despite the extra round of cell divisions in P4.p and P5.p descendants of gonad-ablated eff-1 mutants no vulval differentiation was observed. (M-R) FBF-2::GFP expression, and the corresponding Nomarski images, from the early L3 until the L4 stage. In all panels, anterior is to the left and ventral is to the bottom. Scale bars: in C,L,N and in the inset of J, 10 µm.

 

Figure 3
View larger version (70K):

[in a new window]
  		Fig. 3. fbf-1 and fbf-2 inhibit 1° cell fate specification. Analysis of EGL-17::YFP expression in mid-L3 larvae at the Pn.px or Pn.pxx stage (left side) and in L4 larvae at the Pn.pxxx stage (right side). (A-D) Wild-type, (E-H) gap-1(ga133), (J-M) gap-1(ga133); puf-8 RNAi and (N-Q) fbf-1(ok91) fbf-2(q704); gap-1(ga133) larvae. In all panels, anterior is to the left and ventral is to the bottom. In the graphs, white indicates no EGL-17::YFP expression, grey low expression and black high expression. The arrows in L and P indicate ectopic induction of distal vulval cells; the arrowhead in P indicates an example with expanded EGL-17::YFP expression in VulA and VulB, and the resulting defect in the 2° fate execution. Scale bars: in A,C, 10 µm.

 

Figure 4
View larger version (79K):

[in a new window]
  		Fig. 4. puf-8 regulates the fusion of the distal vulval cells. Vulval cell fusion was analyzed at the Pn.px stage using AJM-1::GFP as a cell junction marker for unfused cells. (A-C) Wild-type, (D-F) puf-8(zh17) single mutants and (G-J) fbf-1(ok91) fbf-2(q704) double mutants. In all panels, anterior is to the left and ventral is to the bottom. In the graphs, white represents fused Pn.px cells, grey indicates fusing Pn.px cells that have started to dissolve their junctions as can be seen for the P8.px cells in G, and black indicates unfused cells with intact AJM-1::GFP-positive junctions. Note that the fraction of unfused cells in fbf-1(ok91) fbf-2(q704) double mutants matches the frequency of ectopically induced distal cells that give rise to the 28% penetrant Muv phenotype (see Table 2, row 13). Scale bar in B: 10 µm.

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2006