spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 26, 2008
doi: 10.1242/10.1242/dev.021196

Development 135, 3321-3323 (2008)
Published by The Company of Biologists 2008
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Harland, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harland, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Induction into the Hall of Fame: tracing the lineage of Spemann's organizer

Richard Harland

Department of Molecular and Cell Biology and Center for Integrative Genomics, University of California, Berkeley, CA 94720-3200, USA.


Figure 1
View larger version (63K):
[in this window]
[in a new window]

  		Fig. 1. Organizer grafts result in induction of a secondary axis. (A) Schematic of the organizer graft created by Spemann and Mangold, using a light-gray newt donor (Triturus cristatus) grafted into a dark-gray host (Triturus taeniatus). The gastrulae are shown in hemisection for illustrative purposes only (dorsal is towards the right, and the dimensions of these embryos are more Xenopus-like than Triturus-like). (B) The famous result of an optimal grafting experiment (Spemann and Mangold, 1924Go), showing a section through the trunk of a twinned embryo. The light-gray graft has contributed to the notochord, medial somite and floor plate of the secondary axis. The graft has an induced neural tube, somites, a pronephros and a secondary archenteron cavity. (C, D) Contemporary organizer grafts from Andrea E. Wills (UC Berkeley, CA, USA). (C) The section shows a rafted organizer labeled with lacZ mRNA and stained with Red-Gal; the section is taken through the trunk of a stage 28 Xenopus laevis embryo, where the axial tissues are also stained with Tor70 antibody. (D) Twinned Xenopus embryo, resulting from an organizer graft carried out at stage 10.

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2008