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Fig. 1. Geminin levels are low during endocycles. Wild-type (A,A') ovariole,
(B,B') stage 13 Drosophila embryo and (C,C') larval salivary
glands stained with (A,B,C, white; A',B',C' inset, green)
 -Geminin and (A', inset, red) --Spectrin
antibodies, and (A',B',C' inset, blue; C', white)
DAPI. (A,A') Geminin is expressed in mitotic follicle
cells of the ovary (stage 1-6), but is absent from endocycling cells after
stage 6 (arrow), with the exception of the polar cells (asterisk). Geminin
levels are downregulated when germline nurse cells enter into the endocycle in
region 3 of the germarium (localized by the -Spectrin antibody
staining; A', inset). (B,B') During embryogenesis,
Geminin expression is shown in mitotic cells, but is absent from endocycling
cells. (C,C') Geminin staining is also absent in the
larval salivary gland, except for the imaginal ring of the salivary gland
(insert, arrow), which contains diploid adult precursor cells.
mr1/mr2 mutant (D,D')
ovariole and (E,E') larval salivary gland, labeled with
(D,E, white; D',E', insets, green) -Geminin antibody and
(D',E', white; D',E', insets, blue) DAPI. Geminin is
ectopically expressed in mr mutant nurse cells and in salivary glands
cells.
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).
The Flipout/Gal4 technique was used to clonally express
UAS-ApcRNAi constructs with GFP.
(B-D''') salivary gland containing (B-B''' and C-C''')
Apc8RNAi or (D-D''') Apc8RNAi;
Apc10RNAi Flipout/Gal4 clones stained with (B,C,D, white;
B''',C''',D''', green) 
