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In ovo time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches

Paul M. Kulesa and Scott E. Fraser

Division of Biology, Beckman Institute 139-74, California Institute of Technology, Pasadena, CA, 91125, USA

This sequence shows the migration of DiI-labeled neural crest cells towards the branchial arches as the embryo undergoes its rotation to one side. Notice how the cells emigrate in streams which spread out to cover a subregion of the periphery.
Duration: 12 hrs.
Time interval between images: 3 min.
* In all the movies, each image represents 10 confocal sections separated by 10 microns each, projected onto 1 image.

Neural crest cells leave from near the midbrain (m), midbrain/hindbrain boundary (m/h) and rostral rhombomeres (r1 and r2) and spread out to cover a wide region adjacent to the neural tube. Duration: 7 hrs
Time interval between images: 3 min

Neural crest cells from r3, r4 and r5 contribute to the stream exiting adjacent to r4. Duration: 3 hrs
Time interval between images: 3 min

Neural crest cells from r3 follow a caudolateral trajectory to join cells exiting adjacent to r4. Duration: 4 hrs
Time interval between images: 3 min

Neural crest cells move into the regions between the streams and make contact with cells from a different stream. In this movie, neural crest cells from the first branchial arch stream migrate caudally towards the second branchial arch. And neural crest cells at the caudal part of the second branchial arch stream meet with cells from the third branchial arch stream. Duration: 2 hrs
Time interval between images: 3 min

We zoom in on the region of neural crest cell interactions lateral to the otic vesicle.
This sequence of images shows neural crest cells from the second and third branchial arch streams interacting. Duration: 3 hrs
Time interval between images: 3 min

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