Fig. 5. Expression of the AIY cell fate marker sra-11::gfp in ttx-3 and ceh-23 mutant animals. sra-11::gfp expression was monitored by mating otIs62 animals that harbor an integrated sra-11::gfp construct with animals of the respective mutant backgrounds. The animals carry a rol-6 injection marker, leading to a slight distortion in the spatial arrangement of neurons. Quantification of the expression data in the AIY interneurons is shown in Table 1. While expression of sra-11::gfp in AVB is strong and highly penetrant in L1 larvae, it is observed less consistently and more weakly in adult animals (compare upper and lower panels; this observation was made with three independently created integrants, otIs62, otIs122 and otIs123). Expression of sra-11::gfp in AVB came to lie out of the plane of focus in the ceh-23(ms23); otIs62 adult animal shown in the lower right panel. Expression of sra-11::gfp in AIA is difficult to consistently detect in larvae, yet clearly visible in adult animals. 64% of wild-type adults show expression (n=45) in AIA and 38% (n=70) of ttx-3(mg158); otIs62 adults show expression in AIA (see lower middle panel).
