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Figure 5


Fig. 5. Expression of notochord and nerve cord markers in two-celled partial embryos. (A) Diagram of the experimental procedure to make two-celled partial embryos. See text for details. (B,D) Two-celled partial embryos isolated before notochord induction. (B) Expression of Not-1 antigen. No expression is observed in either blastomere. (D) Expression of HrETR-1. Both blastomeres show HrETR-1 expression. (C,E) Two-celled partial embryos isolated after notochord induction. (C) Expression of Not-1 antigen. The larger blastomere expresses Not-1. The smaller shows no trace of Not-1 expression. (E) Expression of HrETR-1. The smaller blastomere expresses HrETR-1. The larger one shows no HrETR-1 expression. Scale bar: 100 µm.





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