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Figure 7


Fig. 7. Expression of notochord and nerve cord markers in two-celled partial embryos treated with MEK inhibitor and FGFR inhibitor. (A) Diagram of the experimental procedure to make two-celled partial embryos treated with inhibitors (inh.) of FGF signaling. (B,E,H) Treatment with MEK inhibitor. (B) Not-1 is not expressed in either blastomere. (E,H) Both blastomeres in two-celled partial embryo show HrETR-1 (E) and HrTBB2 (H) expression. (C,F,I) Treatment with FGFR inhibitor. (C) Not-1 is not expressed in either blastomere. (F,I) Both blastomeres show HrETR-1 (F) and HrTBB2 (I) expression. (D,G,J) Treatment with DMSO. (D) The larger blastomere (notochord blastomere) expresses Not-1. The smaller one (nerve cord blastomere) does not. (G) Only smaller blastomere expresses HrETR-1. (J) Only smaller blastomere expresses HrTBB2. Scale bar: 100 µm.





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