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Fig. 3. Comparison of Otx2, Pax2, Pax6 and Six3 expression domains in the OV of Otx-deficient mice. Dark-field images of transverse paraffin sections of the OV of wild-type and Otx mutant embryos hybridised with radioactive probes for Otx2, Pax2, Pax6 and Six3, as indicated. Note how in all genotypes OV have evaginated normally and Pax6 and Six3 expression is similar in all genotypes. In contrast, expression of Pax2 and of the remaining Otx2 allele is extended respectively ventrally and dorsally in both Otx1+/-; Otx2+/- (C, H) and Otx1-/-; Otx2+/- (D-E, I-J) as compared to wild-type (A, F) or Otx1-/- embryos (B, G). Arrows in A-J indicate the limits of expression in the OV. d, dorsal; v, ventral. Bar, 200 µm.





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