Fig. 7. The minimal MCP silencer element. The sequence of the 138 bp minimal MCP silencer element is shown. Putative GAGA and PHO binding sites are boxed. Above the sequence, grey ovals show the approximate locations of protein binding sites deduced from DNA binding studies using embryonic nuclear extracts and in vitro-synthesized proteins. The protein in nuclear extracts that interacts with sequences near GAGA558 is unlikely to be GAF since the binding is not competed by the strong GAGA binding sequence at GAGA548 (see text and Fig. 4). Below the sequence, the extent of the oligonucleotides and the mutated residues are indicated.
