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Fig. 5. Summary of the levels of Hoxd10 expression in whole-mount embryos after transplantation of LS neural tube segments to T regions. Hoxd10 expression was visually scored in donor and host regions of each experimental embryo. This histogram expresses Hoxd10 levels in transplant regions as mean percent of levels (± s.e.m.) in host LS regions (see text). The number of embryos in different experimental groups are given in parentheses. Values are given separately for embryos with transplants placed into anterior and posterior T regions. Stage 14-15 transplant groups (black bars) show high values, irrespective of position. Stage 13 transplant groups (white and gray bars) differ; transplants in anterior T positions show low expression; transplants in posterior T regions show high levels. Within the stage 13 anterior transplant group, transplants + LS3 show a higher mean level value than transplants –LS3. The number of cases are low because the precise level of origin could only be determined for a subset of embryos (see text). Transplants of LS neural tissue + adjacent paraxial mesoderm (gray bar) show a value similar to that of transplants of LS neural tissue alone.





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