Fig. 2. Features of the fwf mutant. (A) Comparisons of a pistil dissected from a anthesis stage Ler flower (a), Ler silique 7 days post-pollination (p), an emasculated unpollinated Ler pistil 7 day post-anthesis (up), compared with a parthenocarpic fwf silique in the Ler background (fwf up) and fwf in the near isogenic line (Col-1) following emasculation (NIL up). A valve has been removed from (a), (p) and (up fwf) to display the presence or absence of seeds. (B) Cleared siliques showing decreased seed set (arrowheads) in proximal region of the fwf silique following cross-pollination with Ler pollen. (C) Section of Ler ovule at anthesis. (D) Section from an unfertilized fwf ovule at anthesis. (E) Ler flower. (F) fwf flower. (G) ctr1-1 flower. (H) ein6 flower. (I-L) Lateral sections of carpel valves examined 7 days post-anthesis. (I) Unpollinated Ler. (J) Pollinated Ler. (K) Unpollinated fwf. (L) Pollinated fwf. (M-P) Transverse sections of carpel valves 7 days post anthesis. (M) Pollinated Ler. (N) Pollinated fwf. (O) Unpollinated fwf from flower below position 30, arrowhead indicates enlarged mesocarp cells. (P) Unpollinated fwf from flower above position 30. Abbreviations: endocarp (e) exocarp (x), mesocarp (m), sclerenchyma (s) and ovule (o). Scale bars: A, 3 mm; C,D, 30 µm; I-P, 50 µm.
