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Fig. 2. SSEA-1 immunostaining on ICMs isolated from blastocysts delayed for 6 days. (A) phase contrast, (B) fluorescence (x40 objective). In situ hybridisation for Sparc mRNA on cultured ICMs from blastocysts delayed for 6 days. (C) gp130+/-, (D) gp130-/- (x10 objective). ICM, proliferating inner cell mass; VE, visceral endoderm; PE, parietal endoderm.





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