Fig. 1. Identification of the first intron in the 5'-UTR of the zebrafish gata1 gene. (A) Structure of the zebrafish gata1 gene. Gray and white boxes denote the coding and noncoding region of the GATA1 cDNA, respectively. E, X and S indicate enzyme sites for EcoRI, XbaI and SpeI, respectively. (B) Southern blot analysis. Zebrafish genomic DNA (lanes 1-3) or GATA1 phage clone DNA (lanes 4-6) was digested with XbaI (lanes 1 and 4), SpeI (lanes 2 and 5) or both enzymes (lanes 3 and 6). After Southern blotting, membrane was hybridized with a probe corresponding to the first intron (black bar in panel A). (C) PCR analysis. PCR was performed using zebrafish genomic DNA (lane 1), GATA1 phage clone DNA (lane 2) and GATA1 cDNA (lane 3) as templates. No template DNA was added in lane 4. Primers are indicated by arrows in A.
