Fig. 3. mShhN is significantly more potent at inducing Dlx and Islet 1/2 in rat E11 telencephalic explants than uShhN. The schematic in the top left-hand corner shows a dorsal view of the E11 rat telencephalon. The blue region delineates the region used in the explant assay for ventral neural induction. The level of induction is indicated by the number of cells expressing Dlx (green), Islet 1/2 (red) or both (yellow). uShhN: A, 3070 nM; B, 384 nM; D, 48 nM; F, 24 nM; H, 12 nM; J, 3 nM. mShhN: C, 48 nM; E, 24 nM; G, 12 nM; I, 3 nM. n=4 for all concentrations except D,E, for which n
25. Note that none of the controls treated with buffer alone contained Dlx- or Islet 1/2-positive cells. D, diencephalon; M, mesencephalon; T, telencephalon.
