spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)


Fig. 9. Immunoprecipitation of radiolabeled Shh (arrow) from the mouse neural cell line C17. 3H-palmitate + 3H-myristate incorporation (lanes 1-4). Lane 1: cytoplasmic fraction from control C17 cell line. Lane 2: cytoplasmic fraction from Shh transfected C17 cell line (Shh-C17). Lane 3: 125,000 g pellet, membrane fraction from control C17 cell line. Lane 4: 125,000 g pellet fraction from Shh-C17. 3H-myristate incorporation (lanes 5,6). Lane 5: 125,000 g pellet fraction from Shh-C17. Lane 6: culture supernatant from Shh-C17. 3H-palmitate incorporation (lanes 7,8). Lane 7: 125,000 g pellet fraction from Shh-C17. Lane 8: culture supernatant from Shh-C17. 35S-methionine+cysteine incorporation (lanes 9-14). Lane 9: cytoplasmic fraction from control C17 cell line. Lane 10: cytoplasmic fraction from Shh transfected C17 cell line (Shh-C17). Lane 11: 125,000 g pellet, membrane fraction from control C17 cell line. Lane 12: 125,000 g pellet fraction from Shh-C17. Lane 13: culture supernatant from control C17 cell line. Lane 14: culture supernatant from Shh-C17 (secreted form).





Right arrow Return to article