Fig. 3. Tsh and not Hth, is a dac repressor in the leg. (A,A') Clonal loss of hth (green in A-C) does not lead to derepression of dac (red in all panels) in the proximal leg (arrows). Ectopic expression of Hth either in flipout clones induced late in development (B,B') or using a dpp-GAL4 driver that is active from early stages onwards (C,C') does not repress dac in the medial leg (arrows in both). In both cases, Hth was detected via a green fluorescent protein (GFP) tag. (D,D') In contrast, ectopic expression of Tsh in the medial leg represses dac (arrows). Clones of cells ectopically expressing Tsh were detected by simultaneously flipping on GFP (blue).
