Fig. 6. Msx1 inhibits mammary epithelial differentiation and upregulates cyclin D1 in cell culture and in vivo. (A) Ribonuclease protection analyses were performed using total RNA obtained from HC11 mammary epithelial cells infected with a control retrovirus (Vector) or with those expressing Msx1 or Msx1A. Infected cells were grown under normal growth conditions (undifferentiated) or in conditions that promote differentiation (differentiated). (B) Ribonuclease protection analyses of total RNA prepared from the mammary fat pads of wild type (WT) and transgenic (TG) mice from virgin or pregnant females at the indicated stages. (A,B) Ribonuclease protection assays were performed using the indicated riboprobes; each lane contains 10 µg of total RNA, except for ß-casein in which 1.5 µg of RNA was used. RPL32 is a control for RNA loading.
