Fig. 6. Asymmetry of dorsal and ventral gene expression is established but not maintained in Dfst7 mutants. Expression of gsc (A-D), chd (E-H), boz (I-L) and bmp2b (M-P) was examined by in situ hybridization at the indicated stages in wild-type and Dfst7 mutants (genotypes shown at the bottom of each panel). In A,B,E,F,I,J,M,N, genotypes were determined by PCR assay after the photographs were taken. (A-L) Animal pole views, dorsal towards the right. (M-P) Lateral views, dorsal towards the right. We obtained similar results to those shown in N and P when we examined bmp2b expression in wild-type embryos co-injected with vox and vent MO.
