Fig. 8 Distinct neural patterning phenotypes in bmp2b/swr mutants and embryos lacking vox and vent function. Expression of dkk1 (A-D), otx2 (E-H), gata2 (I-L), emx1 (M-P) and six3.2 (Q-T) was examined by in situ hybridization in wild-type (A,E,I,M,Q), swr mutant (B,F,J,N,R), Dfst7 mutant (C,G,K,O,S) embryos and MO vox + vent embryos (D,H,L,P,T). Arrowheads in E-H mark the vegetal limit of otx2 expression. (A-D) Shield stage (6 hours), animal pole views, dorsal towards the right. (E-L) 75% epiboly (8 hours), lateral views, dorsal towards the right. (M-T) three-somite stage (11 hours), lateral views, anterior towards the left. In A,B, genotypes were determined by PCR assay after the photographs were taken.
