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Fig. 6. Semi-quantitative analysis of Fgf8b misexpression. Electroporation of pMiwFgf8b at a concentration of 1.0 µg/µl (A,B) and 0.1 µg/µl (C,D) resulted in fate change of the mesencephalic alar plate to the cerebellum. However, electroporation of pMiwFgf8b at a concentration of 0.01 µg/µl resulted in enlargement of the tectum (E,F), as seen with Fgf8a. Electroporation at a concentration of 0.001 µg/µl had no effect (G,H). Embryos were all fixed at E7.5. Scale bars are 4 mm.
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