Fig. 5. Other retinal neuron defects in Math5-/- mice. (A,B) Anti-syntaxin (HPC-1) immunostaining shows that amacrines in vitread INL (arrow) and displaced amacrines in the GCL are grossly unaffected. (C,D) Pax6 is similarly expressed by INL and displaced amacrine cells of wild-type (C) and Math5 mutant mice (D). (E,F) Calretinin-positive A2 amacrines in the INL (arrow) and GCL (arrowhead) are reduced in the Math5 mutant (F) compared to wild-type (E). (G,H) Rod bipolar cells labeled with anti-protein kinase C have cell bodies in the sclerad INL (arrow) and synapse with photoreceptors in the OPL and A2 amacrines in the IPL. These neurons are reduced in Math5-/- retinae and lack well organized synaptic termini in the IPL. (I,J) Anti-vimentin staining shows radial fibers of Müller glia extending across Math5+/+ retinal laminae. The abundance of Müller glia is reduced in Math5-/- eyes. Retinal sections in A-D are from P14 mice and those in E-J are from P21. gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer. The bar is 100 µm in B.
