Fig. 1. Expression of FoxD3 in early Xenopus embryos and comparison to that of Slug. (A-H) Spatial and temporal expression of FoxD3 (A,C,E,G) and Slug (B,D,F,H) analyzed by whole-mount in situ hybridization. (A,B) Early gastrula stage 11 (vegetal view), arrowhead indicates dorsal lip; (C,D) mid-gastrula stage 12; (E,F) late gastrula stage 12.5; and (G,H) mid-neurula stage 16. (I-K) Double-labeled in situ hybridization. (I) Sox2 (light blue) and FoxD3 (indigo), (J) Sox2 (light blue) and Slug (indigo), (K) Zic-r1 (light blue) and FoxD3 (indigo). (C-K) Dorsal views. (L) Histological analysis of FoxD3 distribution at mid-neurula stage. np, neural plate.
