Fig. 3. Amino acid sequence of full-length CRTF. Deduced amino acid sequence (with numbers indicated) from the full-length CRTF cDNA. --- represents the position of the single intron. The poly asparagine (N)- and poly glutamine (Q)-rich runs present in the full-length but not the truncated form of CRTF are shown in red and blue, respectively. The DNA-binding domain (DBD) is boxed. The P1, P2, P3a and P3b peptides are underlined. The H/C residues that may form an atypical zinc-finger motif are in bold. The asterisks denote positions for C-terminal deletions to amino acids 870, 865 and 859 (see Fig. 4). A putative nuclear localization sequence at the C terminus is in green. ^ indicates cloning position of BLAST gene within the DBD for the gene disruption construct.
