Fig. 4. Morphological features of Antirrhinum flowers that express the AFI::DEF/GLO transgenes in a wild-type background. (A-D) Mature flowers displaying various first whorl organ transformations. (A) Lateral view of flower with slightly aberrant petals (pet). (B-D) Frontal view of flowers, with some organs (all petals in B, all petals and stamens in C and all inner organs in D) removed. An anomalous carpel is shown in C. For comparison, B shows a wild-type carpel developing in a flower with a severe first whorl transformation. The position of the dorsal (d), lateral (l) and ventral (v) sepals are indicated. (E,F) SEM of inner epidermal cells within the lobe (E) and tube (F) regions of a petaloid sepal. Bars, 20 µm. (G,H) Expression pattern of DEF (G) and GLO (H) in sequential cross sections from a first whorl organ. The weak sub-epidermal hybridisation signal within the marginal region is most obvious around vascular bundles. Epidermal expression is present in this, and in the adjacent, region. Bars, 200 µm.
