Fig. 1. Egfr in furrow birth and reincarnation. (A) Time line describing critical periods. Initiation of the furrow estimated to occur at 184-186 hours after egg deposition (AED; red line). Two temperature-sensitive points (TSPs) for Egfr: TSP1 is 168-180 hours AED; TSP2 is 192-204 hours AED. (B-D) SEMs: (B) wild type; (C) TSP1 (note only non-ommatidial bristles are present); (D) TSP2 (note normal facets at posterior margin; white arrow). (E-J) Discs labeled with phalloidin, which shows F-actin (red), and -Elav, which shows photoreceptors (green). (E-G) Wild type. (H-J) Upshifted Egfr^tsla/Egfr^topCO. (E) 180 hour AED; before furrow initiation. (F) 192 hours AED; newly initiated furrow and single column of ommatidia. (G) 204 hours AED; further progression of furrow. (H) TSP1 dissected at 192 hours AED; compare with wild type in F. (I) TSP1 dissected at 204 hours AED; furrow still not initiated, compare with wild type in G. (J) TSP2 dissected at 204 hours; birth normal but reincarnation inhibited. Anterior is towards the right. White arrows in F,G,J indicate furrow.

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