Fig. 2. Transformation from neurons and sheath cells to outer support cells in embryos lacking phyl. In this and all the following figures, neurons are stained with anti-Elav antibody (red), sheath cells are stained with anti-Prospero antibody (green), and cells of es organs are stained with anti-Cut antibody (purple). (A,B) Abdominal hemisegments of the PNS in wild type (A), and phyl1/phyl2 (B) embryos. L, lateral region and V, ventral region of the PNS. (C) In the A1(2)29 embryo, the dm region of each abdominal hemisegment contains four outer support cells (blue), two neurons (red), and two sheath cells (green). In total, there are eight Cut-positive cells for these two es organs (E). (D) In phyl2245 /phyl2245 embryos, six outer support cells with one neuron and one sheath cell (left), or eight outer support cells (right) were most frequently observed. (F) In most cases, eight Cut-positive cells are still present (left hemisegment) in phyl2245/phyl2245. Occasionally, four Cut-positive cells were observed (right). Schematic drawings of the two dm es organs are on the left.
