Fig. 3. Misexpression phenotypes of phyl. (A,B) Expression pattern of UAS-GFP/+; Eq-GAL4/+. (A) The wing disk at 3 hour APF. Anterior is to the left. (B) The developing notum at 7 hour APF. At this stage, the notal regions of the two wing disks are attaching to each other to form a complete notum. The arrows indicate the future midline region and anterior is to the top. (C) Wild type notum, and (E) its midline region. (D,F) In the UAS-phyl/+; Eq-GAL4/+ notum, the density of microchaetes increases (D), and is highest in the midline region (F). (G) Wild-type and (H) UAS-phyl/+; dpp-GAL4/+ adult scutella. (I,J) X-gal staining of A101 in wild-type (I) and UAS-phyl/+; dpp-GAL4/+ (J) pupal scutella. Ectopic SOP cells are indicated by arrows in (J). (K,L) Confocal images of the abdominal hemisegments in embryos stained with anti-Elav antibody. (K) Wild-type and (L) sca-GAL4/UAS-phyl. (M,N) Confocal images of the abdominal dm region of a sca-GAL4/ UAS-phyl embryo to reveal the numbers of neurons (red) and sheath cells (green) (M), and the total numbers of es organ progeny (N). (O,Q) In GAL4109-68/UAS-phyl adults, the hair cells and socket cells of the microchaetes are missing (O), and are transformed into neurons (red) and sheath cells (green) (Q). (P) Clusters of one neuron and one sheath cell were seen in wild-type pupal notum.
