Fig. 2. Expression of Dac, Dll and Exd in wild-type and pb5 labial discs. (A) Dac (red) is not expressed in wild-type labial discs. (B) In wild-type labial discs, Dll is expressed in a compact crescent-shaped domain – the distalmost region of the disc. (C) Exd (blue) is present at very low levels in wild-type labial discs. (D,H) The overall pattern of Exd (blue), Dac (red) and Dll (green) in a pb5 labial disc (D) and wild-type (H) T2 leg disc. (E-G) Same labial disc showing Dac, Dll and Exd separately. (E) The Dac (red) domain in pb5 labial discs is relatively narrower that the leg disc (compare with the wild-type T2 leg disc in H). (F) In pb5 labial discs the Dll-expressing domain is larger than wild type and the protein accumulates at levels comparable with those seen in leg and antennal discs.(G) Exd is easily detectable in the proximal portion of the pb5 labial disc. (I-K) Same T2 disc as in H showing Dac, Dll and Exd separately for comparison.
