Fig. 6. The role of Scr in the regulation of appendage genes. (A) Expression of Pb (green) and Scr (red) in a labial disc from an Scr4/Scr5 mutant animal. (B,C) Images of the same disc as in A, showing individual expression patterns. Scr expression is clearly reduced; Pb expression remains unaltered. (D) A Scr4/Scr5 mutant labial disc stained to reveal expression of Dll (red), Exd (blue) and Sal (green). (E-G) Images of separate channels of the same disc as in D. Dll expression is slightly expanded when compared with wild-type (see Fig. 1B); low levels of Exd and little or no Sal (green) expression is detectable, similar to wild-type labial discs.
