Fig. 1. Polyadenylation in maturing mouse oocytes. Radiolabeled 3' UTR of cyclin B1 mRNA, which contains a CPE and polyadenylation hexanucleotide (top) was injected into GV stage oocytes that were then allowed to mature for 16.5 hours (center), or for 3 (GV intact) and 6 (GVBD) hours (right). The RNA was extracted and assessed by denaturing PAGE and phosphorimaging. The approximate size of the newly acquired poly(A) tail is indicted. As a control, a mutated cyclin B1 3' UTR that did not contain a CPE was also injected and the oocytes were analyzed after 16.5 hours of culture. For this experiment, the left panel represents 21 oocytes (-CPE), the middle panel 87 oocytes (+CPE), and the right panel 31 (0 hours), 27 (3 hours) and 25 (6 hours) oocytes. All analyses were performed twice.
