Fig. 1. Identification of aubergine. (A) Schematic of genomic region 32C4-D1 on arm 2L. Left is distal. The positions of restriction sites are shown above the line (B, BamHI; X, XbaI; Xh, XhoI). Arrows indicate start positions and directions of transcription. In the transcripts, the coding regions are shown by boxes. The MR-1 and MR-3 genomic fragments (Schmidt et al., 1999) rescue aub mutant defects. (B) Molecular defects of aub mutant alleles, with the affected amino acids indicated. The frameshift in aub^N11 is caused by a 154 bp deletion which is predicted to add 16 novel amino acids after E740. Two mutants, aub^K86 and aub^N11, share an additional change relative to the wild-type sequence (GenBank Accession Number, X94613), 5549 G to A, which does not affect the protein sequence. Both alleles came from a single mutagenesis (Wilson et al., 1996), and this change presumably represents a polymorphism in the common parental chromosome. The third mutant, aub^HN2, which was obtained in a separate screen using a different parental chromosome (Schüpbach and Wieschaus, 1991) includes three other changes, all of which are 3' to the stop codon mutation: 7304 A to C, 7326 T to A, and 7337 T to C. (C) A Western blot of ovary extracts. A band of approximately 105kDa is recognized in ovary extracts from flies wild-type at the aub locus (w¹¹¹⁸), but not from transheterozygotes for the aub^N11 and aub^HN2 alleles.

Return to article