Fig. 3. Comparison of GFP-Aub and Osk protein distributions in embryogenesis. Each column shows GFP-Aub (A-C), Osk (D-F) and overlap (G-I) at the posterior pole of embryos of increasing age. (A,D,G) Embryo before pole bud formation. GFP-Aub and Osk are found in overlapping sets of particles. Some particles are labeled uniquely with GFP-Aub or Osk, but most show colocalization. (B,E,H) Embryo with pole buds forming. Both GFP-Aub and Osk are cytoplasmic, and continue to colocalize. (C,F,I) Pole cells in a precellularization embryo. GFP-Aub remains cytoplasmic, but some Osk now appears in the nuclei. Within the cytoplasm GFP-Aub and Osk are colocalized in particles of increased size. These particles are frequently concentrated on opposing faces of the nuclei, as indicated by arrowheads. All aspects of Osk protein localization are virtually identical to that displayed by a Vas-GFP fusion protein, with both proteins colocalizing in particles at all stages shown, as well as at later stages of embryogenesis (not shown).
