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Fig. 3. Alae defects in elt-5(RNAi) larvae. (A,C,E) Wild-type; (B,D,F) elt-5(RNAi) larvae. (A,B) Nomarski images of L1 larvae. (A) Wild-type larva, showing normal alae, visible as two ridges along the length of the body (arrowheads). (B) elt-5(RNAi) larva in which alae are absent over most seam cells (short and long arrow) and malformed over two others (box). (C,D) Fluorescence images of the adherens junctions (revealed with JAM-1::GFP) of the larvae shown in A and B, respectively. (C) All seam cells show adherens junctions in the wild-type larva. (D) Some of the seam cells (short arrow) have fused to the epidermal syncytium in the elt-5(RNAi) larva. Some of the unfused seam cells (long arrow) do not have alae. (E,F) Details from A and B, respectively. Areas of detail are marked by the boxes in A,B.





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