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Fig. 4. OTX2 protein level in Otx2{lambda} mutant embryos. (A) Western blot analysis of 7.5 d.p.c. wild-type, Otx2+/-, Otx2{lambda}/Otx2{lambda} and Otx2{lambda}/- embryos, showing that in Otx2{lambda} mutants the OTX2 protein is remarkably reduced. (B) Coomassie Blue staining of the same gel in A. (C) Graphic representation of densitometric scanning indicating a reduction of ~60% of OTX2 protein in Otx2{lambda}/Otx2{lambda} and Otx2{lambda}/- mutants when compared with wild-type and Otx2+/- embryos, respectively.





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