Fig. 5. Quantitative analysis and ribosome affinity profiles of Otx2
mRNA. (A) Total, cytoplasmic and nuclear Otx2 mRNA exhibits a reduction of 
20% when compared with the wild-type Otx2 mRNA. The Otx2 mRNA percentages are the mean of three independent experiments. (B) Ribosome affinity profiles of Otx2, ß-actin mRNAs and in vitro synthesised Otx2 RNA, showing the distribution of the untranslated Otx2 RNA (see also Materials and Methods). (C,D) Ribosome affinity profiles comparing Otx2, Otx2 and ß-actin mRNAs in Otx2/Otx2 embryos at 7.75 (C) and 10.5 d.p.c. (D) show that while more than 50% of the ß-actin and Otx2 mRNAs is detected in the first two fractions, only 25% of the Otx2 mRNA is concentrated in the same fractions. Note that mRNA profiles are not influenced by any quantitative variation of the mRNAs analysed.
