Fig. 3. rib does not function upstream of FGF, DPP or EGFR. All views are lateral. Stage 14 wild-type (A) and rib¹ (B) embryos hybridized with an antisense btl RNA probe. rib mutants have similar expression, except that btl RNA expression is prolonged in TC cells (arrowhead; this difference is more obvious at earlier stages; not shown). Stage 11 wild-type (C) and rib¹ (D) embryos hybridized with an antisense bnl RNA probe show similar expression patterns. Wild-type embryos were co-hybridized with a btl probe (pink staining in C). Stage 12 wild-type (E) and rib¹ (F) embryos stained with anti-KNI have identical expression patterns; KNI is expressed in the DB, LT, and GB, and is lost from the TC and DT cells (arrow). Stage 14 wild-type (G,G') or rho^P38 (H,H') embryos stained with anti-TRH. rho embryos exhibit loss of several DBs (black arrows in H) and have fewer cells in the DT (arrowhead in H), LT (arrowhead in H'), and GB (arrow in H'), when compared with wild type. (Markings in G,G' are identical to H,H', except the view of the wild-type embryo in G is slightly more ventral than in H, placing the DBs out of the plane of focus.) A group of tracheal cells do not invaginate in rho mutants and are found in the same plane of focus as the epidermis (white arrowhead in H'). Early stage 12 wild-type (I) and rho^P38 mutant (J) embryos hybridized with an antisense sal probe show expression in the dorsal cells of tracheal pits (arrow). Embryos were co-hybridized with a salivary gland-specific probe to distinguish mutant from heterozygous embryos (arrowhead in I,J). Wild-type embryo genotypes are as follows: rib¹/CFL hybridized with lacZ (A) or stained with anti-ßgal (E), Oregon R (C), and rho^P38/TUL (G,G',I).

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