Fig. 1. (A) Schematic illustration of hippocampal lamination. So, stratum oriens; sp, pyramidal cell layer (areas CA1 and CA3 are indicated); sr, stratum radiatum; slm, stratum lacunosum-moleculare; ml, dentate molecular layer (oml=outer ml; iml=inner ml); g, granule cell layer; h, hilus; FD, fascia dentata; F, fimbria. (B) Fluorescent microspheres (4 µm diameter) were coated with membrane preparations from cortical cells (P0) and plated on hippocampal slices (P6). After incubation, non-adherent particles were removed. Microspheres adhere only to the stratum oriens, the stratum lacunosum moleculare, the outer molecular layer and the hilar region. Microspheres do not adhere to the pyramidal cell layer, the granule cell layer, and to the stratum radiatum. Bar, 200 µm. (C) Lamina-specific adhesion of membrane-coated fluorescent microspheres to the outer molecular layer of a DAPI-stained hippocampal control slice (P6). Bar, 50 µm. (D) Lamina-specific adhesion of microspheres is abolished on a hippocampal slice (P6) which was treated with hyaluronidase before the adhesion assay. Bar, 50 µm.
