Fig. 6. Western blot for neurocan. Proteins in supernatants from hyaluronidase-treated rat hippocampal slices were separated by gel electrophoresis and analyzed with an antiserum which detects neurocan core proteins (NC-1). Lane 1: Protein size standard. Lane 2: Supernatant after hyaluronidase treatment alone; no signal was detected by the neurocan antiserum. Lane 3: The supernatant from lane 2 was subsequently treated with chondroitinase ABC to remove chondroitinsulfate side chains from CSPGs. After this double enzymatic treatment, a band of approx. 130 kDa, which is characteristic for a neurocan core protein, was detected by the antiserum. Thus, neurocan, substituted with CS, is present in hyaluronidase-treated supernatants from hippocampal slices.
