Fig. 5. Experimental scheme of the rde-1 background RNAi. (A) Control
experiment. Homozygous rde-1 unc-32 animals were microinjected with
med-6 dsRNA, and the total number of eggs laid was counted.
Individual progeny were transferred to individual plates, and phenotypes were
examined after 4 days. (B) Maternal rescue RNAi. Homozygous rde-1
unc-32 animals were microinjected with med-6 dsRNA and were
mated with wild-type males. The total number of eggs laid was counted. Non-Unc
cross progeny were transferred to new plates individually, and checked for
their sex and phenotypes. The percentages of dead embryos were determined by
the differences in the numbers of eggs and viable animals.
