Fig. 2. Generation of Sall1-deficient mice. (A) Targeting strategy of
Sall1-del. Positions of zinc finger motifs are indicated as ovals.
(B) Targeting strategy of Sall1-lacZ. (C) Southern blot analysis of
wild-type (+/+), heterozygous (+/-), homozygous (-/-) Sall1-del
mutant mice. Tail DNA was digested with BamHI and hybridized with
probe B. (D) Genomic PCR of wild-type (+/+), heterozygous (+/-), homozygous
(-/-) Sall1-del mutant mice. The 420 bp band was amplified from
neor gene and the 200 bp band was from Sall1 genome. The
positions of the PCR products are indicated as gray bars. (E) RT-PCR of
Sall1 transcript in wild-type (+/+), heterozygous (+/-), homozygous
(-/-) Sall1-del mutant mice.
