Fig. 1. Primary organoids retain myoepithelial cells and basement membrane. (A)
Detection of keratinpositive and 
-smooth muscle actin-positive cells in
primary mammary organoids at the time of preparation. Frozen sections of
mammary organoids embedded in OCT were immunostained (A) simultaneously with
mouse anti--smooth muscle actin to detect myoepithelial cells (red) and
rabbit anti-keratin antibodies (green) as described in Materials and Methods.
(B) Organoids were stained with rabbit anti-laminin antibodies (green). Scale
bars: 50 µm. DAPI nuclear counterstain is in blue.
