Fig. 3. Growth factors increase the level but not the activation status of
proteinases secreted by primary mammary organoids. Mammary organoids were
treated for 4 to 6 days in collagen I gels with no growth factor (No GF) or
with EGF, HGF, bFGF or KGF. Conditioned medium was collected, concentrated and
run on substrate zymograms. (A) Gelatin zymogram. Latent gelatinase B (LGB),
active and latent gelatinase A (AGA, LGA) and a high molecular weight
gelatinase (HG) were detected. (B) Casein zymogram. A band corresponding to
the molecular weight of latent stromelysin-1 (LStr1) was detected. (C)
Plasminogen casein zymogram. A band corresponding to the molecular weight of
uPA was detected. There were no differences in the activation status of the
gelatinases, caseinase or plasminogen activator between growth factor treated
organoids and untreated controls.
