Fig. 4. Abnormal elongation and migration of Pax6 mutant
(rSey2/rSey2) EGL cells co-cultured with
wild-type cells. (A) PKH26-labeled wild-type and Pax6 mutant EGL
cells from E21 embryos were mixed with P2 wild-type EGL cells at a ratio of
1:20 and cultured in cellular reaggregates. (B,D) Wild-type cells elongate
long axons (B), which occasionally display T-shaped morphology (D). (C,E)
Mutant cells rarely extend long neurites (C), often forming randomly
misoriented dwarfed processes (E). Cultures were maintained for 40 hours (B,C)
and 72 hours (D,E), respectively. Scale bars: 40 µm in B,C; 20 µm in
D,E.
