Fig. 5. Cell-autonomous effect of Pax6 on elongation of bipolar parallel
fibers. EGL cells in microexplant cultures were transfected with either GFP
cDNA alone (wt, rSey2) or GFP with Pax6 or Pax6-EnR cDNAs
(rSey2+Pax6, wt+Pax6-EnR). (A) Morphology of granule cells shown by
GFP fluorescence (green) together with immunofluorescence with Zic1 (red) and
Pax6 (blue). Scale bar: 20 µm. (B) The length (left) and the number (right)
of neurites quantified in GFP expressing cells. Each column in the right graph
represents the number of neurites as indicated.
(*P<0.001, **P<0.0001, in
comparison with the wild-type GFP control; ***P<0.07 in
comparison with the mutant GFP control).
